A speedy transcription activator-like effector nuclease (TALEN) preparation protocol was developed based on widely used Golden Gate TALEN and TAL Effector Kit 2.0. This new protocol can be used to prepare any custom 18-bp binding TALENs in about 12 hours, which is faster than CRISPR. The results of the study are published in ACS Publications.
According to the research article, the protocol involves the use of a set linear monomers, a final TALE-FokI backbone plasmid, and a pipeline to assemble the ready-to-use TALEN expression plasmid, which were all designed for the study. The TALEN construction pipeline can simply acquire several positive colonies with high efficiency of over 80 percent. Results of the tests involving five NF-κB genes showed that the new protocol has high efficiency, reproducibility, reliability, and applicability. Furthermore, it was shown that the fabricated TALEN has better editing efficiency than CRISPR.
Get more details about the protocol in ACS Publications.
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